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. 2011 Sep 9;20(12):1971–1981. doi: 10.1002/pro.734

Figure 2.

Figure 2

Preparation of CDR3-grafted proteins. (A) Amino acid sequences of recombinant proteins. CDR3 sequences of D2-L19 (1RI8) and cAb-Lys2 (1RJC) are in ocher. CDR3 sequence of cAb-CA05 replaced by CDR3 of D2-L19 or cAb-Lys2 is in pink. Grafted CDR3 sequences of cAb-CA05-(1RI8) and cAb-CA05-(1RJC) are in blue. The Ubi loop replaced by CDR3 sequences is orange, and His68 of Ubi is green. Grafted CDR3 sequences of Ubi-(1RI8) and Ubi-(1RJC) are cyan. H68C in Ubi-(1RI8) and Ubi-(1RJC) is red. (B) Cation-exchange chromatography of refolded cAb-CA05-(1RI8). (C) Cation-exchange chromatography of Ubi-(1RI8). (D) SDS-PAGE of purified proteins. Molecular size by marker is indicated at right. (E) Reverse-phase HPLC of cAb-CA05-(1RI8). (F) Reverse-phase HPLC of Ubi-(1RI8).