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H-D exchange with Ubi and Ubi-(1RI8). Plots of three amino acid residues around the CDR3 grafted root −3 to +6: (A)5V; CDR3-1, (B)13I; CDR3+1, (C)16E; CDR3+4. (D)29K; exist in α-helix that is a portion of the scaffold. Ubi (•), Ubi-(1RI8) (▴). Peak intensity (%) is the relative peak intensity ratio for each measurement compared with the corresponding peak intensity of the initial measurement.
