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Cleavage of the fluorescent substrate Dabcyl-EHVKLVE-EDANS by cell-associated Sapp1p. Panel A: cleavage products obtained from incubation of C. parapsilosis cells with the substrate. Panel B: substrate cleavage products from incubation of C. parapsilosis cells in the presence of 1 μM pepstatin A. Arrows indicate the position of Sapp1p-specific cleavage product. The position of the cleavage product was verified by in vitro substrate cleavage with purified Sapp1p.
