Figure 10.

Accelerated axonal regeneration of BACE1 KO axons. A, Above is a schematic of a reciprocal sciatic nerve transplantation between BACE1 KO and WT. Below is a representative image of a nerve that was transplanted using this nerve graft technique. The arrows point to where the transplanted segment was sutured in. B, WT nerve segment transplanted into a BACE1 KO host nerve. BACE1 KO axons regenerated through the WT graft well. The arrows point to regenerated BACE1 KO axons. C, BACE1 nerve segment transplanted into a wild-type host. A few WT axons grow through BACE1 graft (arrow). Scale bars: B, C, 10 μm. D, BACE1 KO DRG explants had axons 119.0 and 150.0% longer on average than those of WT DRG explants at 2 and 4 d after culture. BACE1 inhibitor IV (50 nm) increased axonal outgrowth of WT DRG explants by 98.0% at 4 d after culture. Values are mean ± SEM. *p < 0.001.