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. 2012 Mar 15;23(6):1058–1067. doi: 10.1091/mbc.E11-10-0852

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© 2012 Berens and Toczyski. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 1: — Colocalization of Mec1-Ddc2 and Mrc1 promotes Rad53 phosphorylation independent of Ddc1 and Dpb11. (A, B) Strains with or without a LacO array and with the indicated combination of Mrc1-LacI, Ddc2-LacI, and Ddc1-LacI under the control of Gal promoter were grown in raffinose and arrested in nocodazole for 3 h. Galactose was pulsed for 1 h before transcription was inhibited with dextrose, and then samples were collected at the indicated time points from galactose addition and blotted for Rad53 and the LacI fusion proteins. (C) As in A and B, except that strains were grown at 23°C, arrested with nocodazole, and either kept at 23°C or shifted to 34°C at 1 h before galactose induction.