Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2012 Mar 15;23(6):1058–1067. doi: 10.1091/mbc.E11-10-0852

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2012 Berens and Toczyski. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

“ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society of Cell Biology.

PMC Copyright notice

FIGURE 4: — Rad53 phosphorylation in the absence of 9-1-1 requires Mrc1. (A) Cells of the indicated genotype were treated with HU for 2.5 h at 30°C. All strains carry the sml1-1 mutation, which suppresses lethality of mec1∆. Rad53 phosphorylation was visualized by SDS–PAGE and Western blot. Cdc28 served as a loading control. (B) After HU treatment as described in A for the indicated time, HU was washed out and cells were plated on rich medium to measure viability. (C) Cells of the indicated genotype were treated with HU for 2.8 h at 30°C and processed as in A. (D) Cells of the indicated genotype were treated with MMS for 4 h at 22.5°C, and phosphorylation of FLAG-tagged Rad9 and Rad53-HA was visualized by SDS–PAGE and Western blotting.