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. 2012 Mar 15;23(6):1068–1079. doi: 10.1091/mbc.E11-09-0745

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© 2012 Stevens and Page-McCaw. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 5: — Mmp1 is required for leading-edge cell migration. (A, B) Aspect ratios of wild-type (A, A′) and Mmp1 (B, B′) leading-edge cells were calculated by measuring perpendicular axes (shown in red) at 0 h (A, B) and 5 h (A′,B′) after wounding. See Materials and Methods for details. (C) Aspect ratio analysis for wild type (black bars) and Mmp1 mutants (blue bars). Black stars indicate that wild-type cells at 5 h are significantly different from wild-type cells at 0 h postwounding (p < 0.0001) by Student's t test. Blue stars show that Mmp1-mutant cells are significantly different from wild type at 5 h (p = 0.0001) postwounding by Student's t test. There is no significant difference in aspect ratio between wild-type and Mmp1 mutant cells 0 h postwounding, and similarly cells in Mmp1 mutants show no significant change in aspect ratio between 0 and 5 h after wounding. (D) Aspect ratio 5 h postwounding. Leading-edge cells in Mmp1 mutants (p = 0.0001), A58>Mmp1-dsRNA (p = 0.0015), A58>Timp (p = 0.041), and A58>Bsk^DN (p = 0.0043) are significantly less elongated than wild type at 5 h postwounding. Numbers in the base of each bar (C, D) denote the number of cells measured. Error bars represent SEM. (E, F) Actin-GFP localization around control A58>Actin-GFP (E) and mutant Mmp1² A58>actin-GFP (F) wounds (n = 3 for each genotype). White arrows (E) indicate actin-rich protrusions into the wound bed. Asterisk indicates a wound bed (A, B, E, and F). All scale bars, 20 μm; A and B′ scale bar in A′; E and F scale bar in F.