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. 2012 Mar 15;23(6):1080–1095. doi: 10.1091/mbc.E11-07-0588

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© 2012 Zwaenepoel et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 6: — Ezrin Y477F fails to promote Eps8 protein activities. Immunofluorescence was performed on LLC-PK1 cells coexpressing ezrin Y477F and (A) GFP-Eps8 or (B) GFP-Eps8L1a with an anti-VSVG antibody to detect ezrin (red). No morphological changes are observed in cells expressing both proteins. (A) Eps8 remains localized at the tip of the microvilli (inset, right). (B) Eps8L1a is diffuse in the cytoplasm and absent from the tip of the microvilli (inset, right). Bars, 10 μm; inset, 1 μm.