Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2012 Mar 15;23(6):1095–1103. doi: 10.1091/mbc.E11-08-0668

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2012 Urbé et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

“ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society of Cell Biology.

PMC Copyright notice

FIGURE 7: — USP21 is required for primary cilium formation. A549 cells were transfected with siRNA specific to USP21 (OL1, OL4) or nontargeting siRNA (NT1) and serum starved for 48 h to induce primary cilium formation. (A) A maximal projection of NT1 and USP21-targeting, siRNA (OL1)-transfected cells is shown after staining of acetylated α-tubulin as a marker for primary cilia (Ac Tub, green) and pericentrin (PCNT, red). DNA was stained with DAPI (blue). Scale bar, 10 μm. (B) The percentage of cells with a primary cilium (∼800 cells counted per condition) is significantly reduced for cells transfected with siRNA specific to USP21 (error bars, SD; n = 3 independent experiments).