Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2012 Mar 15;23(6):1115–1128. doi: 10.1091/mbc.E11-09-0783

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2012 Saitoh et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

“ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society of Cell Biology.

PMC Copyright notice

FIGURE 1: — Distribution of phosphorylated SR proteins in adult mouse testis. (A) The distribution of phosphorylated SR proteins and expression of Ranbp2 are developmentally regulated. Representative views of cross-sections of seminiferous tubules stained with the mouse monoclonal antibody SC35 (a), DAPI (b), and anti-Ranbp2 (c). (d–f) Merged images of SC35 and DAPI. (e, f) Enlarged pictures of the boxed areas in d. SC35 antigens were present in a speckled pattern in the nucleus of spermatocytes (e), whereas they formed granular structures in the cytoplasm in the spermatids (f). The differentiation stage was determined based on morphology under DAPI staining. (B) An adult mouse testis section was immunostained with SC35 (green) and antibodies against Ddx4 for nuage/germinal granules (red). Nuage/germinal granules (arrows) were not recognized by SC35 (a–c). In addition, cells with SC35-stained granules (arrowheads) did not form nuage/germinal granules (d–f). Bars, 20 μm (A, a–d), 10 μm (A, e–f, and B). (C) Immunoblot analysis of cell lysate prepared from adult mouse testis. Antibody SC35 recognizes mouse srsf2 (asterisk) in this tissue, but it fails when lysate is treated with phosphatase. Histone H3 serves as a loading control.