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. 2012 Mar 15;23(6):982–995. doi: 10.1091/mbc.E10-10-0855

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© 2012 Murata et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 5: — Abnormal egg morphology and eggshell permeability of the piga-1(tm2939) embryos. (A) Wild-type embryo. (B–F) piga-1(tm2939) embryos. (B) Apparently normal embryo. (C) Spherical embryo. (D) Embryo with protruding eggshell (arrow). (E) Embryo with constriction in the middle. (F) Embryo bent in the middle. (G) Eggshell permeability of the wild-type and the piga-1(tm2939) embryos. The lipophilic dye FM4-64 stains cell membranes. In wild-type N2 and rescued piga-1(tm2939) embryos, only eggshells and polar bodies were stained. In the piga-1(tm2939) embryos, their eggshells were permeable to FM4-64, and the cell membranes of the embryos were stained. Bars, 20 μm.