Skip to main content
. 2012 Mar 13;7(3):e32199. doi: 10.1371/journal.pone.0032199

Table 1. Bacteria and plasmids used in this study.

Bacteria Description Source
H. influenzae
R2866 Blood isolate from an immunocompetent child with meningitis immunized with the Hib vaccine. A.L. Smith
R2866 ΔvapBC-1 The vapBC-1 TA locus replaced with a kanamycin resistance cassette. This work
R2866 Δfis The fis gene replaced with a chloramphenicol resistance cassette. This work
R2866 ΔfisΔvapBC-1 fis and vapBC-1 double mutant. This work
E. coli
BL21 F dcm ompT hsdS(rB mB ) gal [malB +]K-12S) EMD Bioscience
BL21(DE3) F ompT hsdS (rB mB ) gal dcm λDE3 EMD Bioscience
DD12 MC4100 recA::RP4-2-Tc::Mu KmR [23]
DH5α F endA1 glnV44 thi-1 recA1 relA1 gyrA96 deoR nupG Φ80dlacZΔM15 Δ(lacZYA-argF)U169, hsdR17 Lab collection