Table 1. Bacteria and plasmids used in this study.
Bacteria | Description | Source |
H. influenzae | ||
R2866 | Blood isolate from an immunocompetent child with meningitis immunized with the Hib vaccine. | A.L. Smith |
R2866 ΔvapBC-1 | The vapBC-1 TA locus replaced with a kanamycin resistance cassette. | This work |
R2866 Δfis | The fis gene replaced with a chloramphenicol resistance cassette. | This work |
R2866 ΔfisΔvapBC-1 | fis and vapBC-1 double mutant. | This work |
E. coli | ||
BL21 | F− dcm ompT hsdS(rB − mB −) gal [malB +]K-12(λS) | EMD Bioscience |
BL21(DE3) | F− ompT hsdS (rB −mB −) gal dcm λDE3 | EMD Bioscience |
DD12 | MC4100 recA::RP4-2-Tc::Mu KmR | [23] |
DH5α | F− endA1 glnV44 thi-1 recA1 relA1 gyrA96 deoR nupG Φ80dlacZΔM15 Δ(lacZYA-argF)U169, hsdR17 | Lab collection |