Table 2. Primers for fis and vapBC-1 cloning.a .
FisXhoIfor | 5′-ATAACTCGAGATGTTAGAACAACAACG-3′ |
FisKpnIrev | 5′-AAACGGTACCTTATCCCATACCG-3′ |
D99NFor | 5′-[Phos]AATCTATGGATCGCTTGTCACG-3′ |
D99NRev | 5′-[Phos]ATTATTTCCGATAGGTCGCC-3′ |
VapCSacFor | 5′-CGAGGAGCTCTATGATTTATATGTTAGACACC-3′ |
VapCXhoIRev | 5′-GAATCTCGAGTTTTGTCCAATCTTGCC-3′ |
BCFor | 5′-GCTTTCTAGACAGGCTAAATATACCG-3′ |
BCRev | 5′- GGTCTCTAGAGGCATTGTGCGCCAC -3′ |
Fis28Sacfor | 5′-AAAAGAGCTCAATGTGCCAGTGACC-3′ |
Fis28Xbarev | 5′-AATATCTAGACCGTTAAGGCATCAGCAGG-3′ |
Fis28Xbafor | 5′-CGTGTCTAGAATATGCTTGGTATCAACCG-3′ |
Fis28Pstrev | 5′-GCACCTGCAGTTTACTGGAAAAGTGC-3′ |
CmNhefor | 5′-GCAGGCTAGCCCGACGCACTTTGC-3′ |
CmNherev | 5′-CTTAGCTAGCTTACGCCCCGCCCTG-3′ |
BC1403for | 5′-ACTAGAATTCATCATTTACTCATTGACTTGC-3′ |
BC1403rev | 5′-GTTAGGATCCTGAAACACTTTAGTAAGC-3′ |
1403Xbafor | 5′-ATTTTCTAGAGTTATTGTCTCATGAGCGG-3′ |
1403Xbarev | 5′-TTCCTCTAGAGAAATACGGGCAGACATGG-3′ |
Restriction enzyme sites used for cloning are underlined.