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. 2012 Mar 13;7(3):e32199. doi: 10.1371/journal.pone.0032199

Table 2. Primers for fis and vapBC-1 cloning.a .

FisXhoIfor 5′-ATAACTCGAGATGTTAGAACAACAACG-3′
FisKpnIrev 5′-AAACGGTACCTTATCCCATACCG-3′
D99NFor 5′-[Phos]AATCTATGGATCGCTTGTCACG-3′
D99NRev 5′-[Phos]ATTATTTCCGATAGGTCGCC-3′
VapCSacFor 5′-CGAGGAGCTCTATGATTTATATGTTAGACACC-3′
VapCXhoIRev 5′-GAATCTCGAGTTTTGTCCAATCTTGCC-3′
BCFor 5′-GCTTTCTAGACAGGCTAAATATACCG-3′
BCRev 5′- GGTCTCTAGAGGCATTGTGCGCCAC -3′
Fis28Sacfor 5′-AAAAGAGCTCAATGTGCCAGTGACC-3′
Fis28Xbarev 5′-AATATCTAGACCGTTAAGGCATCAGCAGG-3′
Fis28Xbafor 5′-CGTGTCTAGAATATGCTTGGTATCAACCG-3′
Fis28Pstrev 5′-GCACCTGCAGTTTACTGGAAAAGTGC-3′
CmNhefor 5′-GCAGGCTAGCCCGACGCACTTTGC-3′
CmNherev 5′-CTTAGCTAGCTTACGCCCCGCCCTG-3′
BC1403for 5′-ACTAGAATTCATCATTTACTCATTGACTTGC-3′
BC1403rev 5′-GTTAGGATCCTGAAACACTTTAGTAAGC-3′
1403Xbafor 5′-ATTTTCTAGAGTTATTGTCTCATGAGCGG-3′
1403Xbarev 5′-TTCCTCTAGAGAAATACGGGCAGACATGG-3′
a

Restriction enzyme sites used for cloning are underlined.