Figure 3. All seven TFs are continuously required for OR expression.

(A) Schematic of the TARGET experiments. Flies were shifted at late pupal stage from 18°C to 29°C (red line), or from 29°C to 18°C (green line); the RNAi was induced specifically at 29°C. (B–D) Or92a and Or47b in situ hybridizations (red) counterstained with DAPI (blue). (B) With the suppression of RNAi at 18°C, the OR was expressed in all genotypes (red staining). (C) The TF knock down at the end of pupal development (shift from 18°C to 29°C) fully suppresses OR expression. (D) Developmental TF knock down (shift from 29°C to 18°C) does not affect OR expression except for zf30c-IR.