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. 2012 Mar 13;7(3):e33418. doi: 10.1371/journal.pone.0033418

Figure 2. Lactate activates HIF-1 in normoxic endothelial cells.

Figure 2

HUVECs were incubated during 24-h with 10 mM lactate or not. (A) The transcriptional activity of HIF-1 was determined using a dual luciferase reporter assay in HUVECs. *p = 0.0207; n = 4. (B) VEGF protein level was measured using ELISA in the supernatant of cells. ns, non-significant; n = 6. (C) bFGF protein level was measured using ELISA in the supernatant of cells. ***p<0.0001; n = 5. (D) VEGFR2 protein expression was detected by western blot in cell lysates. **p = 0.0084; n = 4–5. (A–D) Error bars reflect mean ± SEM.