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. 2012 Mar 13;6(3):e1564. doi: 10.1371/journal.pntd.0001564

Figure 1. Vaccine antigen production.

Figure 1

(A) Sm-TSP-2 was ligated to a pET41a vector backbone in-frame with the C-terminal 6×His tag, expressed in E. coli.by autoinduction and purified by IMAC followed by CEX chromatography and desalting into PBS. 1 = uninduced soluble fraction; 2 = autoinduced soluble fraction; 3 = purified protein. (B) Sm-TSP-2/5B was ligated to a pET41a vector backbone in-frame with the C-terminal 6×His tag, expressed in E. coli.by IPTG induction and purified by IMAC and desalting into PBS. 1 = uninduced soluble fraction; 2 = IPTG-induced soluble fraction; 3 = purified protein.