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. 2012 Mar 13;7(3):e33230. doi: 10.1371/journal.pone.0033230

Figure 2. Expression of the H2bYFP fusion does not alter the ability of MHV68-H2bYFP to establish infection.

Figure 2

A) Limiting dilution PCR analysis to determine the frequency of viral genome positive splenocytes from mice infected with 1,000 pfu of the indicated virus and harvested at 16 days post-infection. B) Limiting dilution analysis to determine the frequency of splenocytes capable of reactivating virus. Serial dilutions of splenocytes were plated on MEF indicator monolayers, and the presence of reactivating virus was determined by the presence of cytopathic effect (CPE). C) Representative flow plots showing the gating strategy to determine the percentage of EYFP and H2bYFP positive B cells. D) Quantitation of the percentage of EYFP and H2bYFP positive B cells at the indicated days post-infection from mice infected with the 1,000 pfu of the indicated virus. Results shown in panels A and B are from 3 individual experiments with 3 to 5 mice per group. Error bars represent the standard error of the mean. Each symbol in panel D represents a single mouse, and the horizontal bars represent the mean. Results are from 3 independent experiments with 3–5 mice per group.