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. 2012 Mar 9;7(3):e33144. doi: 10.1371/journal.pone.0033144

Figure 5. PTB alters Ago2 association of mRNAs in HeLa cells.

Figure 5

(A) PTB and nPTB was simultaneously knocked down in triplicates in HeLa cells and Ago2 was immunoprecipitated from control and PTB/nPTB siRNA transfected cells. PTB, nPTB, Ago2 expression was followed by Western hybridization. Tubulin was used as loading control. *: non-specific band detected with the nPTB antibody. (B–F) q-PCR analysis of mRNAs which association with Ago2 is modulated by PTB. RNAs were isolated from control and PTB/nPTB siRNA transfected cells and from Ago2 IPs obtained from the same cells. RNAs were quantified and normalized with GAPDH RNA. The data show the relative abundance of the normalized RNAs compared to the control siRNA transfected cells and the Ago2 IP from the same cells. Error bars represent the standard deviation of three independent experiments (A). *: p<0.05, **: p<0.001.