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. 2012 Mar 9;7(3):e33144. doi: 10.1371/journal.pone.0033144

Figure 6. PTB and let-7 miRNA contribute together to regulate gene expression in C. elegans.

Figure 6

(A) Synchronized L1 animals were placed at semi-permissive temperature (20°C) and adult animals were scored after seventieth-two hours. The animal sterility observed in the population is caused by either a vulval bursting at the L4-adult transition or by a severe gonadal defect. Error bars represent the 95% confidence interval from independent experiments (n) where between 20 and 40 animals have been scored. ***: p<0.0001 (B) let-7 level remained unchanged in the let-7ts/ptb-1 animals. RNAs were purified from the indicated genotypes and probed for let-7 and U6 RNAs. The amount of RNA was used for Northern blotting is indicated on the top of the panel and the U6/let-7 ratios are presented at the bottom of the panel.