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. 2012 Mar 9;7(3):e33356. doi: 10.1371/journal.pone.0033356

Figure 4. Effect of siRNA-mediated silencing of ZFP36 on T cell activation, proliferation, and survival.

Figure 4

PBMCs from healthy donors were transfected by nucleofection with non-specific siRNA (NC siRNA) or ZFP36-specific siRNA (ZFP36 siRNA). A. Expression of CD25 and CD69 in CD4+ and CD8+ T cells was measured 24 h after stimulation with anti-CD3/CD28. Dotted lines: un-stimulated cells; solid lines: stimulated cells; grey lines: NC siRNA-transfected cells; black lines: ZFP36 siRNA-transfected cells. B. CFSE dilution of labeled CD4+ and CD8+ T lymphocytes. Dotted lines: cells transfected with NC siRNA; solid lines: cells transfected with ZFP36 siRNA. C. Cell cycle status of ZFP36 siRNA-transfected T cells. Cells were stained with PI. Solid lines: cells transfected with NC siRNA; dotted lines: cells transfected with ZFP36 siRNA. D. Apoptotic rates of ZFP36 siRNA-transfected T cells as determined by Annexin V staining. Percentages represent the frequency of Annexin V+ cells within the CD4+ and CD8+ T cell populations of PBMCs transfected with NC siRNA or ZFP36 siRNA.