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. 2012 Mar 9;7(3):e32400. doi: 10.1371/journal.pone.0032400

Figure 4. Body temperature and detection of viral RNA in the nasal cavity after infectious challenge.

Figure 4

Pigs were vaccinated with the adjuvanted split (solid line), non-adjuvanted whole (dashed line) or mock (dotted line) vaccine, and all pigs were subsequently challenged with infectious Eng195 influenza virus. (A) Rectal temperature (in °C) was monitored one day prior and everyday following challenge. Results are expressed as the mean value ± SEM for each vaccination group. *, P<0.01. (B) Nasal swabs were collected one day prior and everyday following challenge. Shedding was measured in all samples through the detection of viral RNA by a modified influenza A M gene real-time RT-PCR (RRT-PCR) assay. By the use of a standard curve (on each test plate) generated from a dilution series of infectious Eng195 virus, the relative equivalent unit (REU) of viral RNA was determined based upon the cycle threshold (Ct) value obtained for each sample. Results are expressed as the mean REU ± SEM for each vaccination group.