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. 2012 Mar 9;7(3):e32080. doi: 10.1371/journal.pone.0032080

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Ehrlund et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Experimental design. (B) qPCR of Control and siSF ± cAMP-treated cells show approximately 70% efficiency of SF-1 RNAi-mediated knockdown on mRNA level. (C) Western blot showing almost complete knockdown of SF-1 protein after RNAi-treatment. StAR, a protein under transcriptional control of SF-1, is also depleted, showing efficiency of knockdown on target gene level. (D) Venn diagram showing overlap between the different groups of differentially expressed genes.