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. 2012 Mar 9;7(3):e32080. doi: 10.1371/journal.pone.0032080

Figure 5. Putative SF-1 target genes in TGFβ signalling pathways identified from pathway enrichment analysis in the DAVID online resource, siSF-1 effect on H295R cell proliferation.

Figure 5

(A) qPCR showing the effect on mRNA level of SF-1 knockdown ± cAMP treatment proteins in the TGFβ-pathway. (B) Flow-cytometry analysis of cells treated with TGFβ2 show significantly more cells in S-phase and less in G1/G0 after TGFβ2 treatment. (C) Schematic representation of the TGFβ-pathway with genes changed by SF-1 knockdown marked red for upregulation and green for downregulation. (D) Flow cytometry analysis of siSF-1 or control treated cells show more cells in G1/G0-phase and less in S-phase after SF-1 knockdown. (E) The SF-1 gene expression profile can classify malignant from benign adrenocortical tumors in clinical samples. The heatmap shows relative expression of genes differentially expressed in our siSF-1 microarray experiments in a study cohort of 58 ACCs and 34 ACAs [53]. Up- and downregulation is indicated by red and green, respectively, scaled across rows. The top bar illustrates the significant (p<0.01) separation of carcinomas (red) from adenomas (blue) by hierarchical clustering. Error bars show standard deviation. Statistical analysis done with Student's t-test (* p<0.05; **p<0.01; ***p<0.001).