Figure 3. Immunoreactivity of 213Bi-2556 mAb for gp41 and determination of its Ka by Scatchard analysis.
a) gp41 ELISA of 2556 mAb radiolabeled with high specific activity 213Bi (5 mCi/mg). Binding of 213Bi-2556 to gp41 was compared to that of unlabeled 2556 mAb with (2556-CHXA″) or without (2556 standard) attached CHXA″ ligand. 213Bi-2556 was stored with human serum albumin (HAS) radioprotector (2556-Bi/HAS) or without it (2556-Bi); b) Scatchard plot of 213Bi-labeled 2556 and 246D mAbs binding to HIV-infected hPBMCs.