Figure 4. Targeting and killing of HIV-infected cells in vitro with ²¹³Bi-2556 mAb.

a) binding of “cold” 2556 to chronically-infected ACH-2 cells by flow cytometry. HIV-1-negative A3.01 cells were used as controls; b) killing of HIV-infected hPBMCs with ²¹³Bi-2556 mAb. 246D is an anti-gp41 mAb and 1418 is a negative control; c) killing of chronically-infected ACH-2 cells with ²¹³Bi-2556 mAb. Stimulated - PMA stimulated ACH-2 cells, unstimulated – unstimulated ACH-2 cells. The unlabeled antibodies were present in the same amounts as the radiolabeled antibodies.