Table 2. Strains and plasmids used in this study.
Strain or Plasmid | Relevant Characteristic(s) | Source or Reference |
P. carotovorum subspp. carotovorum | ||
LMG 2410 | Isolated from Cucumis sativus | BCCM |
LMG 2412 | Isolated from Hyacinthus orientalis | BCCM |
LMG 2442 | Isolated from Brassica oleracea | BCCM |
LMG 2444 | Isolated from Solanum tuberosum (tuber soft rot) | BCCM |
LMG 2913 | Isolated from soil | BCCM |
P. atrosepticum | ||
LMG 2374 | Isolated from Apium graveolens var. dulce | BCCM |
LMG 2375 | Isolated from Solanum tuberosum (tuber soft rot) | BCCM |
LMG 2386 | Isolated from Solanum tuberosum (stem rot) | BCCM |
LMG 2391 | Isolated from soil | BCCM |
SCRI 1043 | Isolated from Solanum tuberosum (tuber soft rot) | [37] |
Plasmids | ||
pJexpress411 | Kanr, cloning/expression vector, T7 promoter | this study |
pJexpress404 | Ampr, cloning/expression vector, T5 promoter | this study |
pET21-a(+) | Ampr, cloning/expression vector, T7 promoter | Novagen |
pJMPC1 | Kanr, pJexpress411 with Pectocin M1 inserted into NdeI/XhoI sites | this study |
pJMBPR | Kanr, pJexpress411 with Pectocin M2 inserted into NdeI/XhoI sites | this study |
pETMPCI | Ampr, pET21-a(+) with Pectocin M1 inserted into NdeI/XhoI sites | this study |
pETMPCID222A | Ampr, pET21-a(+) with Pectocin M1 D222A inserted into NdeI/XhoI sites | this study |
pETMBPR | Ampr, pET21-a(+) with Pectocin M2 inserted into NdeI/XhoI sites | this study |
BCCM = Belgian Co-ordinated Collections of Micro-organisms, NCPPB = National Collection Plant Pathogenic Bacteria, SCRI = Scottish Crop Research Institute.