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. Author manuscript; available in PMC: 2013 Mar 13.
Published in final edited form as: Biochemistry. 2012 Mar 1;51(10):2113–2121. doi: 10.1021/bi3001038

Table 1.

Iron release kinetics from H318A sTFR complexes.a

Complex k1C (min−1) k2N (min−1) k1N (min−1) k2C (min−1)
Fe2hTF/sTFRb 5.5 ± 0.9 1.4 ± 0.2 2.8 7.2
Fe2hTF/H318A sTFR 1.8 ± 0.7 1.5 ± 0.2 1.7 ± 0.1 5.9 ± 0.9
Complex k1 (min−1)
Conformational Change 		k2C (min−1)
Fe3+ Release
FeChTF/sTFRb 20.6 ± 1.2 7.2 ± 0.4
FeChTF/H318A sTFR - 5.5 ± 0.6
Complex k1 (min−1)
Conformational Change 		k2N (min−1)
Fe3+ Release
FeNhTF/sTFRb 22.0 ± 0.7 1.7 ± 0.6
FeNhTF/H318A sTFR 17.4 ± 2.4 1.1 ± 0.1
a

Averages and 95% confidence intervals for kinetic runs performed on N=3–5 different days. Each day 3 kinetic traces were averaged before fitting.

b

From reference 12.