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. Author manuscript; available in PMC: 2013 Mar 13.
Published in final edited form as: Biochemistry. 2012 Mar 1;51(10):2113–2121. doi: 10.1021/bi3001038

Table 2.

Iron release kinetics from [H475A,H684A] sTFR complexes.a

Complex k1C (min−1) k2N (min−1)
Fe2hTF/sTFRb 5.5 ± 0.9 1.4 ± 0.2
Fe2hTF/[H475A,H684A] sTFR 3.1 ± 0.1 1.2 ± 0.1
Complex k1 (min−1)
Conformational Change 		k2C (min−1)
Fe3+ Release
FeChTF/sTFRb 20.6 ± 1.2 7.2 ± 0.4
FeChTF/[H475A,H684A] sTFR 16.7 ± 2.2 7.5 ± 0.9
Complex k1 (min−1)
Conformational Change 		k2N (min−1)
Fe3+ Release
FeNhTF/sTFRb 22.0 ± 0.7 1.7 ± 0.6
FeNhTF/[H475A,H684A] sTFR 15.3 ± 0.3 1.7 ± 0.1
a

Averages and 95% confidence intervals for kinetic runs performed on N=3–5 different days. Each day 3 kinetic traces were averaged before fitting.

b

From reference 12.