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. Author manuscript; available in PMC: 2013 Mar 13.

Published in final edited form as: Biochemistry. 2012 Mar 1;51(10):2113–2121. doi: 10.1021/bi3001038

Table 3.

Iron release kinetics from sTFR Δ757–760 complexes.^a

Complex	k_1C (min⁻¹)	k_2N (min⁻¹)
Fe₂hTF/sTFR^b	5.5 ± 0.9	1.4 ± 0.2

	k₁ (min⁻¹) Conformational Change	k₂=k_1N (min⁻¹)	k₃=k_2C (min⁻¹)

H349A Fe₂hTF/sTFR^c	23.7 ± 4.6	6.7 ± 0.3	0.61 ± 0.02
Fe₂hTF/sTFR Δ757–760	89.4 ± 2.0	5.2 ± 0.2	1.0 ± 0.1

Complex	k₁ (min⁻¹) Conformational Change	k_2C (min⁻¹) Fe³⁺ Release

Fe_ChTF/sTFR^a	20.6 ± 1.2	7.2 ± 0.4
H349A Fe_ChTF/sTFR^d	9.2 ± 0.8	1.4 ± 0.5
Fe_ChTF/sTFR Δ757–760	48.9 ± 3.7	3.2 ± 0.1

Complex	k₁ (min⁻¹) Conformational Change	k_2N (min⁻¹) Fe³⁺ Release

Fe_NhTF/sTFR^a	22.0 ± 0.7	1.7 ± 0.6
Fe_NhTF/sTFR Δ757–760	31.9 ± 0.5	3.4 ± 0.1

^a

Averages and 95% confidence intervals for kinetic runs performed on N=3–5 different days. Each day 3 kinetic traces were averaged before fitting.

^b

From reference 12.

^c

From reference 15.

^d

From reference 17.