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. Author manuscript; available in PMC: 2013 Apr 1.
Published in final edited form as: Cell Microbiol. 2012 Jan 9;14(4):500–516. doi: 10.1111/j.1462-5822.2011.01735.x

Figure 2. Deletion of Smt1 produces a strain that make only un-methylated GlcCer.

Figure 2

Analysis of specific lipids extracts of WT, Δsmt1, Δsmt1+SMT1, Δgcs1, and Δgcs1+GCS1 cells along with the structure illustration of α-OH-Δ4-Δ8-ceramide, α-OH-Δ4-Δ8,9-methyl-ceramide, α-OH-Δ4-Δ8,9-methyl-GlcCer and α-OH-Δ4-Δ8-GlcCer. The WT along with Δsmt1+SMT1 reveals almost identical amounts of α-OH-Δ4-Δ8,9-methy-GlcCer but negligible amounts of α-OH-Δ4-Δ8-ceramide and its methylated product α-OH-Δ4-Δ8,9-methyl-ceramide. However, the Δsmt1 does not produce methylated GlcCer and accumulates α-OH-Δ4-Δ8-ceramide. In contrast, Δgcs1 accumulated α-OH-Δ4-Δ8,9-methyl-ceramide and lacked methylated and unmethylated GlcCer. All results were means ± SD of three independent experiments. ≠P<0.05, Δsmt1 versus wild-type; ¥P<0.05, Δgcs1 versus WT.