Fig. 1.

The inhibitory effect on [³H]-MPP⁺ apical uptake by Caco-2 cells cultured in high glucose (HG cells) versus cultured under 5.5 mM (control cells). Osmolarity control was performed with mannitol. Confluent Caco-2 monolayers were preincubated at 37 °C for 60 min with Hanks’ buffer and then incubated with 200 nM [³H]-MPP⁺ for 5 min. Each value represents the mean ± SEM of the triplicates of two independent experiments (n = 6). * P < 0.05 in comparison to control cells