. 2010 Mar 6;62(1):23–29. doi: 10.1007/s10616-010-9257-1

Table 1.

[³H]-MPP⁺ apical transport kinetics across Caco-2 cells under 5.5 mM (control cells) and high glucose (HG cells)

	Km (μM)	V_max (nmol/mg protein/min)	Kd (pmol/mg protein/min/μM)
Caco-2	43.9 ± 2.0^*	0.30 ± 0.01	2.6 ± 0.01^*
HG Caco-2	88.1 ± 4.2^*	0.28 ± 0.01	2.0 ± 0.01^*

Substrate binding affinity (Km), maximum transport capacity (V_max) and diffusion coefficient (Kd) for this substrate were determined by nonlinear regression analysis. Each value represents the mean ± SEM of the triplicates of at least three different experiments (n = 12). The values followed by (*) in each column are significantly different at P < 0.05