FIG. 4.

The majority of neurospheres derived from the injured cortex are from GFP-expressing cells. (a) A 50% stretch injury leads to a greater increase in GFP expression compared to control in both the hippocampus (left panels) and cortex (right panels) of P8 mice (scale bar 300 μm). (b) The GFP intensity above a threshold limit is significantly greater in injured hippocampus (left) and cortex (right) at 6 and 72 h following a 50% stretch injury (p<0.01, Student t test; n=8 slices from two experiments). (c) The number of neurospheres per well in flow cytometry sorted GFP-positive and negative cells from the injured hippocampus is equivalent (n=3 experiments). (d) The number of neurospheres per well in flow cytometry sorted GFP-positive cells is significantly greater than GFP-negative cells from the injured cortex (p<0.01, Student t test; n=3 experiments).