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. Author manuscript; available in PMC: 2012 Sep 1.
Published in final edited form as: Nat Genet. 2012 Feb 19;44(3):348–351. doi: 10.1038/ng.1090

Figure 4. Inhibition of FGF and Hedgehog signaling in palatal explants demonstrate their activatory and inhibitory roles in rugal stripe maintenance respectively.

Figure 4

Pattern of rugae, visualized by Shh in situ hybridization in palatal shelf explants cut posterior to ruga 2 and cultured for 24 (a-c, g-i) and 48 (d-f, i-l) hours. (a-f) Culture with the FGF inhibitor SU5402 shows reduced levels of Shh expression in treated explants (c,f) relative to controls (a,b,d,e). (g-l) Culture with the Hedgehog inhibitor cyclopamine shows increased levels of Shh expression in treated explants after 24 hours (i) relative to controls (g, h). Levels of Shh expression then fall after 48 hours culture with Cyclopamine (l) compared to controls (j,k). All specimens oriented with anterior to right, medial up. Scale bar = 200 μm.