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. Author manuscript; available in PMC: 2012 Sep 15.
Published in final edited form as: J Immunol. 2012 Feb 15;188(6):2794–2804. doi: 10.4049/jimmunol.1102068

Figure 6. SPUL40 signal enhances cell surface expression of gpUL18.

Figure 6

Cell surface expression of gpUL18 was monitored by flow cytometry on HFFF cells infected for 72 h with the RAds indicated. RAd-UL18 (100 PFU/cell) plus RAd-CTRL (30 PFU/cell) and RAd-UL18 plus RAd-UL40 (30 PFU/cell) were used as standards in all panels. RAd-UL18 plus a second RAd (30 PFU/cell) encoding: (A) SPUL40 cloned in-frame with RFP, (B) SPHLA-A2 in-frame with RFP, (C) UL40 with residues 24-37 replaced with residues 12-24 from HLA-A2, (D) UL40 with residues 1-14 replaced with first 2 residues from HLA-C, (E) UL40 with HLA-E-binding peptide replaced with an HIV-1 GAG epitope, (F) strain Toledo UL40, (G) UL40 with the M16T mutation, or (H) strain 3157 UL40. Results are representative of three independent experiments.