Figure 4.

Defective osteoclast / osteocyte TRAP expression and increased apoptosis (TUNEL positive) in OA osteocytes: A representative TRAP stained section shows an increased in the number of osteoclasts (a, i-ii). Increased TRAP positive osteocytes were observed in OA subchondral bone (a, iii-iv). A representative TUNEL stained section shows that more number of OA osteocytes are undergoing apoptosis compared to controls. Arrows represent TUNEL positive osteocytes and arrow heads represents live/nuclear stain (b, i-ii). b, iii is the bar graph showing increased TUNEL positive OA osteocytes. Bars equal mean±SD, n=5, *p≤0.05.