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. Author manuscript; available in PMC: 2012 Mar 14.
Published in final edited form as: Front Biosci (Elite Ed). 2011 Jun 1;3:1315–1325. doi: 10.2741/e335

Figure 4.

Figure 4

Sanggenon C inhibits proteasome activity in vitro and in cultured H22 and P388 cells. (A and B) Inhibition of chymotrypsin-like activity by Sanggenon C. Purifed human 20S proteasome or H22 cell lysate (10 microgram protein) was treated with different doses of Sanggenon C for 90 mins and chymotrypsin-like activity was detected by a fluorescence plate reader. The IC50 values of Sanggenon C were 4 microM, 15 microM each for 20S proteasome and H22 cell lysate. (C and D) Does effect on proteasome inhibition and PARP cleavage. Murine H22 cells (C) and P388 cells (D) were separately treated with either solvent DMSO (DM) or indicated concentrations of Sanggenon C for 6 h, followed by Western blotting analysis using specific antibodies against ubiquitinated proteins (Ub), p27 and PARP. Molecular weight of p27 is 27 kDa,. Full length PARP is 116 kDa, and the cleaved fragment of PARP is 85 kDa. GAPDH was used as a loading control.