Figure 1. The effect of pyrvinium on Wnt signaling during basal, osteogenic and chondrogenic differentiation of MSCs in vitro.
Representative Western blot analysis and the quantification of the relative β-catenin protein levels in the cytoplasmic fractions of MSCs normalized to β-actin. Decreased β-catenin levels were identified in the cytoplasmic fractions of pyrvinium-treated MSCs committed towards osteogenic (B) and chondrogenic lineages (C) when compared to the untreated cells and the cells treated with the control compd 211; the similar effect was also identified in the undifferentiated cells (A). The statistical significance between experimental groups and control was determined by ANOVA followed by Tukey’s multiple comparison test; ***p<0.0005, **p<0.005, and *p<0.05, n =4.