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. 2012 Feb 28;8(4):430–441. doi: 10.7150/ijbs.3632

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© Ivyspring International Publisher. This is an open-access article distributed under the terms of the Creative Commons License (http://creativecommons.org/licenses/by-nc-nd/3.0/). Reproduction is permitted for personal, noncommercial use, provided that the article is in whole, unmodified, and properly cited.

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A. Changes in expression of MTHFR (green) and HCY (red) protein contents, by immunohistochemistry analysis, in hearts from Sham, myocardial infarction-induced (MI), sham-operated and treated with H₂S (Sham+ H₂S), and myocardial infarction-induced and treated with H₂S (MI+ H₂S) mice. B. Bar graph of changes in integrated optical density (IOD) in expression of MTHFR and Hcy in Sham, MI, Sham+H₂S, and MI+ H₂S mice heart tissues. The micrographs were taken under the identical set of conditions for all groups. C and D. Changes in expression of CSE and CBS proteins contents, by Western blot analysis, in hearts from Sham, myocardial infarction-induced (MI), sham-operated and treated with H₂S (Sham+ H₂S), and myocardial infarction-induced and treated with H₂S (MI+ H₂S) mice. Examples of Western blot images of the proteins studied and contents of β-actin in the respective samples and results of the Western blot analysis, relative protein expression is reported as ratio of integrated optical density (IOD) of each band to the IOD of the respective β-actin band. * P < 0.05 vs. Sham, Sham+H₂S, and MI+H₂S. # p<0.05 vs. MI. n=6 for all group.