Fig 2.

Induction and repair of DSBs after 2 hours of etoposide and doxorubicin treatment. The A7 (FLNa+) and M2 (FLNa−) cells were treated with 10 μM etoposide or 0.25 μg/ml doxorubicin for 2 h, and then the drug was removed and the cells were kept in drug-free medium. The cells were fixed at various time points for γH2AX immunostaining. The numbers of γH2AX nuclear foci were counted from 100-150 cells in each slide, and the experiment was repeated twice. Panels A and B show the average numbers of γH2AX nuclear foci after etoposide (Panel A) or doxorubicin (Panel B) treatment (* p<0.05, ** p<0.01). Error bars are standard deviations. Panel C and D show the exponential regression analysis. After DNA damage reached highest level (0 hour for etoposide and 6 hour for doxorubicin), the numbers of γH2AX against the repair time were shown in X-Y scattered plot (Diamond and Square). Solid lines are the exponential regression fitting. The exponential regression equations along with the coefficient of determination, R-squared (R²), are shown in the figure.