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. Author manuscript; available in PMC: 2013 May 1.
Published in final edited form as: Cancer Lett. 2011 Dec 11;318(1):99–105. doi: 10.1016/j.canlet.2011.12.007

Fig. 1.

Fig. 1

RNA interference-mediated silencing of TRPM7 did not induce apoptotic cell death. BxPC-3 and PANC-1 cells transfected with anti-TRPM7 siRNA or non-targeting control siRNA were incubated with FITC-conjugated annexin V and PI, and then analyzed for apoptosis by flow cytometry. The cells undergoing early apoptosis (lower right) and late apoptosis (upper right) are enclosed within the black border, with the proportion of apoptotic cells as indicated. The baseline level of apoptotic cells in the control siRNA-transfected cells may be attributed to electrical pulsation during Nucleofection®, detachment of cells by trypsinization, and disaggregation of cells by filtration prior to flow cytometric analysis. This experiment was repeated twice with similar results.