Figure 7. Sperm count and testosterone level of Tas1r1/mCherry knock-in mice.

[A] Total number of caudal epididymal sperm in Tas1r1 null-mutant mice. Number of sperm in the caudal part of the epididymis were counted in male wild-type [+/+], heterozygous [+/−] and homozygous [−/−] mutantTas1r1 animals with identical strain background. Data are mean values ±SEM of 17–46 animals of the three genotypes. [B] Serum testosterone levels in Tas1r1-deficient male mice. Testosterone concentrations were measured in 4–6 month old male littermates of wild-type [+/+], heterozygous [+/−] and homozygous [−/−] Tas1r1 mice by a commercial enzyme-linked immunoassay. Data, expressed as means ± SEM, are obtained from 3 animals of each genotype with triplicate determinations; statistical analysis was done by a paired T-test; a p-value of ≤0.05 was considered to be statistically significant.