Skip to main content
. 2012 Feb 29;7(2):e32354. doi: 10.1371/journal.pone.0032354

Figure 8. Morphology of Tas1r1-null sperm from the Tas1r1/mCherry mouse line.

Figure 8

[A] Analysis of sperm morphology of wild-type and Tas1r1-deficient sperm. Isolated epidydymal sperm from C57BL/6 wild-type animals [+/+] and Tas1r1-deficient mice [−/−] were fixed, stained with Coomassie blue and subsequently subjected to bright field light microscopy. [B and C] Quantitative morphometric analysis of the sperm head of Tas1r1-deficient mice. To quantify dimensions of the sperm head, the length from the tip of the acrosome to the sperm neck ([I]) and to the post-acrosomal region ([II]) was scaled; in addition, circumference of sperm head ([III]) and the area of the whole sperm head ([IV]) were determined (for overview s. [B]). Data represent mean values ± SEM of the determined parameter which were obtained from 5 Tas1r1-deficient (black bars) and wild-type animals (grey bars); 8–15 sperm from each preparation were analyzed.