Table 1.
Experimental evidence related to the role of basophils and DC in induction of Th2 immunity.
| Reference | Methods employed | Conclusions |
|---|---|---|
| [6] | OVA-papain immunization model Basophil depletion mediated by MAR-1 DC manipulation in CD11c-DTR model |
Basophils express MHC-class-II and costimulatory molecules DCs are not required for the development of papain-induced Th2 responses DCs are not able to induce CD4+ Th2-cells in vitro except if basophils are included in the culture |
|
| ||
| [8] |
T. muris-infected mice and injection of S. mansoni eggs Basophil depletion mediated by MAR-1 DC manipulation in CD11c-DTR model |
Basophils express MHC-class-II and IL-4 Depletion of basophils in vivo impairs immunity to T. muris Minimal induction of Th2 cytokines in mice in which MHC-class-II expression was restricted to CD11c+ cells. Th2 cytokines production was restored with a neutralizing monoclonal antibody to interferon-γ |
|
| ||
| [7] | S. venezuelensis-infected mice | Basophils secrete IL-4 and express CD80, CD86, CD62L, and MHC class II Basophils induce antigen-specific Th2-cells in vitro in the absence of DC Enhanced Th2 responses result when basophils are cultured with antigen-specific IgE |
|
| ||
| [48] |
N. brasiliensis infection Basophil depletion mediated by anti-Thy-1.2 mAb |
In the absence of IL-4 and/or IL-13-producing T cells, basophils contribute to efficient worm expulsion |
|
| ||
| [28] |
S. mansoni infection and S. mansoni egg injection Basophil depletion mediated by MAR-1 DC manipulation in CD11c-DTR model |
Depletion of DCs results in impaired CD4+ T cell production of Th2 cytokines; depletion of basophils has no effect |
|
| ||
| [29] |
N. brasiliensis infection Basophil depletion mediated by MAR-1 |
Depletion of basophils does not diminish the development of IL-4 producing CD4+ T cells Basophils recruitment into the dLN depends on IL-3 |
|
| ||
| [23] | OVA-papain immunization model and N. brasiliensis infection Basophil-deficient mice Mcpt8Cre and DC-ablated mice |
Basophils are not required for in vivo priming of Th2-cells in N. brasiliensis-infected mice or after immunization with OVA-papain, with constitutive deletion of basophils DC-ablated mice are impaired in mounting a Th2-cell response against OVA-papain despite normal basophil recruitment |