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. 2012 Mar 4;2012:804592. doi: 10.1155/2012/804592

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Copyright © 2012 Chengcheng Gao et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Real-time quantitative RT-PCR analysis of Dickkopf-1 mRNA expression in 20 pairs of human gastric cancer and adjacent noncancerous human gastric tissues. (a) Gel images of electrophoresis. “N” refers to noncancerous gastric tissues; “GC” refers to gastric cancer tissues. (b) The average level of Dikkopf-1 mRNA expression in gastric cancer tissues was significantly higher than that in noncancerous gastric tissues (P < 0.001). GAPDH gene was used as an internal control. (c) The specificity of Dikkopf-1 antibody was analyzed by western blot testing. A single band of 35 kDa was detected in gastric cancer tissues. β-actin antibody was used as control with the single band of 42 kDa.