Table 4.

Observed and theoretical % helical content of NSP4_112-140-caveolin-1 peptide-peptide interactions.

	N-Cav2-20		Cav19-40		Cav68-80		C-Cav161-178
	Observed	Theoretical	Observed	Theoretical	Observed	Theoretical	Observed	Theoretical
Aqueous buffer	21.7 ± 0.8	23.6 ± 2.6	23.8 ± 1.2	24.4 ± 0.6	24.2 ± 0.3	23.9 ± 0.7	25.0 ± 1.7*	35.3 ± 1.6
50% TFE	46.3 ± 7.2	47.6 ± 1.8	58.7 ± 7.7	56.3 ± 2.3	45.9 ± 2.8	49.8 ± 1.8	44.2 ± 10.6*	67.8 ± 3.2
1 mM SUV	34.7 ± 6.0	37.7 ± 1.9	37.7 ± 3.6	39.0 ± 2.3	31.3 ± 0.2*	37.5 ± 1.9	51.9 ± 2.4	49.5 ± 6.2

NSP4_112-140-Caveolin-1 Peptide-Peptide Interactions: Percent α-helical content of NSP4_112-140 peptide + Caveolin-1 peptides, in aqueous buffer (10 mM potassium phosphate buffer), 50% trifluoroethanol (TFE) and in the presence of 1 mM SUV (55 : 35 : 10, POPC/cholesterol/DOPS). Observed values were compared with theoretical values, and those that were statistically significant by a Student's t-test (P < 0.05; denoted by an asterisk) were considered structurally altered, indicative of an interaction. Results are presented as means ± S.D. (n = 3 or 4).