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. 2012 Mar 14;7(3):e31931. doi: 10.1371/journal.pone.0031931

Figure 2. Expression analysis of shuffled promoters in tobacco protoplast transient assay.

Figure 2

Fifty shuffled-promoters randomly selected from each of the six libraries (LssF, LssFS, LmsFSF, LmsFFS, LhsFuasFScp and LhsFSuasFcp) were fused with GUS reporter gene. These shuffled promoter-GUS constructs were evaluated along with promoter-GUS constructs of CaMV35S and parent (F and FS) promoters in tobacco protoplast transient assay as described in “Materials and Methods”. The average GUS activity (n mole MU/min/mg protein) of three replicates was presented in the histogram.