Figure 2. Effect of the overproduction of YoeBsl and YefM/YoeBsl complexes in E. coli SC36 (MG1655 ΔyefM-yoeB).

A) Cells transformed with control plasmid (pFUS2), with the plasmid carrying yoeBsl (pFUS2-Tox), or with the plasmid carrying the yefM/yoeBsl (pFUS2-TA) were grown in liquid LB medium to mid-logarithmic phase. At this time (time zero), 0.2% glucose (diamond) was added to one half of each culture and 0.2% arabinose (square) to the other half. Cell growth was monitored measuring the OD₆₀₀ of the cultures at different times. The means and standard deviation of three different experiments is presented. B) 5 µl of serial dilutions of the different cultures, taken one hour after protein induction, were inoculated in LB plates and incubated overnight at 37°C. The upper plates were inoculated with cells from media with glucose (repression), and the lower plates were inoculated with cells from media with arabinose (induction).