Figure 4. EMSA assays of the 173 bp intergenic region upstream of the yefM/yoeBsl operon with different proteins: (A) purified complex YefM/YoeB-His₆sl (0, 1, 2 and 5 µM); (B) purified YefM-His₆sl (0, 2, 4 and 8 µM); (C) Purified YoeB-His₆sl (0, 1, 2, and 4 µM).

D) EMSA assays with in vitro reconstituted YefM-His₆/YoeB-His₆sl complexes. The absence or presence of YoeB-His₆sl, and/or YefM-His₆sl is indicated by −/+ respectively. 2 µM of YoeB-His₆sl were used (lanes 3–7), and mixed with increasing amounts (1, 2, 4 and 8 µM) of YefM-His₆sl (lanes 4–7). 1 µM of natural purified YefM/YoeB-His₆sl complex was used as a control (lane 8). E) EMSA assays of different promoters from S. coelicolor (the SCO number is indicated) with (+) or without (−) 1 µM of the purified complex YefM/YoeB-His₆ sl.