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. 2012 Mar 14;7(3):e33245. doi: 10.1371/journal.pone.0033245

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Brun et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Cultured human keratocytes (panel A) and conjunctival fibroblasts (panel B) were exposed for 2 minutes to APCP with or without a 5 mM-pretreatment with NAC and subsequently cultured under optimal conditions. The externalization of phosphatidylserine was analyzed by flow cytometry after double staining of the cells with Annexin-V-FITC and propidium iodide, collecting at least 10,000 events. Single or double positive cells are expressed as a percentage of fluorescent intensity. In cells pre-treated with NAC, the increased percentage of AnnexinV/PI positive cells at 2 and 6 hours post-treatment returned to control values after 12 hours. Data are expressed as mean ± SE (error bars) of at least two independent experiments.